Blanco de calcoflúor: en búsqueda de un mejor diagnóstico
DOI:
https://doi.org/10.22517/25395203.25151Keywords:
Calcofluor white; KOH; Mycological culture; Diagnosis; Fungi.Abstract
Objective: calcofluor white (CFW) is a fluorescent stain that allows observing fungal structures in different clinical samples thanks to its affinity for chitin. Darkfield microscopy facilitates the correct visualization of pathogens, favoring patients' timely and correct diagnosis. therefore, this work aims to evaluate the capacity for identifying mycotic structures in different biological samples of CFW staining.
Materials and methods: thirty-six biological samples (vaginal fluid, bronchoalveolar lavage, cerebrospinal fluid, scales, urine, cornea, blood culture, and biopsy) were evaluated for fungi. All samples were processed by the three techniques: potassium hydroxide 20% (KOH), mycological culture and CFW.
Results: KOH technique gave a positive result in 58.3% of the cases, culture in 69.4% and CFW staining in 72.2%. The sensitivity and specificity of the CFW technique against KOH were 95% and 67%, while against mycological culture was 100% and 91%.
Conclusions: this study demonstrates that the BCF technique is a suitable method for identifying fungal structures in clinical specimens because it showed high sensitivity and specificity relative to the traditional method and culture.
Downloads
References
Ramírez LCC, Lozano LC. Principios físicoquímicos de los colorantes utilizados en microbiología. Nova. 2020; 18(33): 73-100.
Morales-Restrepo N, Cardona-Castro N. Métodos de diagnóstico en micología. CES Medicina. 2018; 32(1): 41-52.
Hageage GJ, Harrington BJ. Use of calcofluor white in clinical mycology. Laboratory Medicine. 1984; 15(2): 109-12.
Sánchez-Armendáriz K, Fernández-Martínez RF, Moreno-Morales ME, Villegas-Acosta L, Meneses-González F, Arenas-Guzmán R. Sensibilidad y especificidad del examen directo micológico con blanco de calcofluor para el diagnóstico de onicomicosis. Medicina Cutánea Ibero-Latino-Americana. 2013; 41(6): 261-6.
Mourad B, Ismail M, Hawwam S, Msseha M, Hassan R. Evaluation of the efficacy of fluorescent staining and chicago sky blue staining as methods for diagnosis of dermatophytosis in hair and nails. Clinical, Cosmetic and Investigational Dermatology. 2019; 12: 751.
Bagga B, Vishwakarma P, Sharma S, Jospeh J, Mitra S, Mohamed A. Sensitivity and specificity of potassium hydroxide and calcofluor white stain to differentiate between fungal and Pythium filaments in corneal scrapings from patients of Pythium keratitis. Indian Journal of Ophthalmology. 2022; 70(2): 542.
Li L, Zhang S, Pradhan S, Ran Y. Facial Sporotrichosis with Liver Cirrhosis Detected by Calcofluor White Treated with Itraconazole. Mycopathologia. 2021; 186(1):141-2.
Shi B, Xia Z, Tang W, Qin C, Cheng Y, Huang T, et al. Rapid Diagnosis of IPA Relied on Calcofluor White Fluorescence Staining: Two Cases Report. Journal of Tropical Pediatrics. 2021; 67(1): fmaa115.
Bonifaz A, Rios-Yuil JM, Arenas R, Araiza J, Fernández R, Mercadillo-Pérez P, et al. Comparison of direct microscopy, culture and calcofluor white for the diagnosis of onychomycosis. Revista iberoamericana de micología. 2013; 30(2): 109-11.
Idriss MH, Khalil A, Elston D. The diagnostic value of fungal fluorescence in onychomycosis. Journal of Cutaneous Pathology. 2013; 40(4): 385-90.
Mikulska M, Furfaro E, Viscoli C. Non-cultural methods for the diagnosis of invasive fungal disease. Expert Review of Anti-Infective Therapy. 2015; 13(1): 103-17.
Abdelrahman T, Letscher-Bru V, Waller J, Noacco G, Candolfi E. Dermatomycosis: comparison of the performance of calcofluor and potassium hydroxide 30% for the direct examination of skin scrapings and nails. J Mycol Méd. 2006; 16: 87-91.
Bao F, Fan Y, Sun L, Yu Y, Wang Z, Pan Q, et al. Comparison of fungal fluorescent staining and ITS rDNA PCR‐based sequencing with conventional methods for the diagnosis of onychomycosis. Journal of the European Academy of Dermatology and Venereology. 2018; 32(6): 1017-21.
Pihet M, Le Govic Y. Reappraisal of conventional diagnosis for dermatophytes. Mycopathologia. 2017; 182(1): 169-80.
Weinberg JM, Koestenblatt EK, Tutrone WD, Tishler HR, Najarian L. Comparison of diagnostic methods in the evaluation of onychomycosis. Journal of the American Academy of Dermatology. 2003; 49(2): 193-7.
Petinataud D, Berger S, Ferdynus C, Debourgogne A, Contet‐Audonneau N, Machouart M. Optimising the diagnostic strategy for onychomycosis from sample collection to fungal identification evaluation of a diagnostic kit for real‐time PCR. Mycoses. 2016; 59(5): 304-11.
Tangarife-Castaño VJ, Flórez-Muñoz SV, Mesa-Arango AC. Diagnóstico micológico: de los métodos convencionales a los moleculares. Medicina y Laboratorio. 2015; 21(5-6): 211-42.
Wiegand C, Bauer A, Brasch J, Nenoff P, Schaller M, Mayser P, et al. Are the classic diagnostic methods in mycology still state of the art? JDDG: Journal der Deutschen Dermatologischen Gesellschaft. 2016; 14(5): 490-4.
Chen B, Li W, Pang Y, Zhang N, Bian S, Liu C, et al. Rapid detection of fungi from blood samples of patients with candidemia using modified calcofluor white stain. Journal of Microbiological Methods. 2021; 184: 106202.
Goenka C, Lewis W, Chevres‐Fernández LR, Ortega‐Martínez A, Ibarra‐Silva E, Williams M, et al. Mobile phone‐based UV fluorescence microscopy for the identification of fungal pathogens. Lasers in Surgery and Medicine. 2019; 51(2): 201-7.
Abastabar M, Mosayebi E, Shokohi T, Hedayati MT, Amiri MRJ, Seifi Z, et al. A multi-centered study of Pneumocystis jirovecii colonization in patients with respiratory disorders: Is there a colonization trend in the elderly? Current Medical Mycology. 2019; 5(3): 19-25.
Downloads
-
Vistas(Views): 16
- PDF (Español (España)) Descargas(Downloads): 19
Published
How to Cite
Issue
Section
License
Cesión de derechos y tratamiento de datos
La aceptación de un artículo para su publicación en la Revista Médica de Risaralda implica la cesión de los derechos de impresión y reproducción, por cualquier forma y medio, del autor a favor de Facultad de Ciencias de la Salud de la Universidad Tecnológica de Pereira. 1995-2018. Todos los derechos reservados ®
por parte de los autores para obtener el permiso de reproducción de sus contribuciones. La reproducción total o parcial de los trabajos aparecidos en la Revista Médica de Risaralda, debe hacerse citando la procedencia, en caso contrario, se viola los derechos reservados.
Asimismo, se entiende que los conceptos y opiniones expresados en cada trabajo son de la exclusiva responsabilidad del autor, sin responsabilizarse ni solidarizarse, necesariamente, ni la redacción, ni la editorial.
Es responsabilidad de los autores poder proporcionar a los lectores interesados copias de los datos en bruto, manuales de procedimiento, puntuaciones y, en general, material experimental relevante.
Asimismo, la Dirección de la revista garantiza el adecuado tratamiento de los datos de carácter personal