Mathematical Model for Analyzing the Fluorescence Emission of Biological Tissue
DOI:
https://doi.org/10.22517/23447214.25544Keywords:
Optical fluorescence spectroscopy, Precancerous tissue, Mathematical modelAbstract
Optical emission fluorescence spectroscopy allows for determining biochemical changes in normal and pathological biological tissue in-vivo or in biopsies. The aim of the study is to analyze the chemical and physical properties of the cervix tissue. The mathematical model is presented to examine and observe the fluorescence emission of the tissue. In the precancerous state development, the optical properties of the tissue can be altered not only by the light dispersion and the fluorescence increase in the epithelium but for the fluorescence reduction in the stroma. Beer-Lambert Law was used to describe light propagation in the tissues. Four components of cervix tissue were identified: collagen, elastin, NADH, and flavins. It was possible to generate support for the medical diagnosis of precancerous lesions on cervix tissue by applying the model with developed algorithms. A good fit of the spectrum was obtained and predictions to the model were properly made, with significative differences for each fluorophore parameter, especially on collagen and NADH.
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