Cuantificacion de taxol y baccatin iii en callos de taxus baccata por cromatografia liquida de alta eficiencia (hplc)


Authors

  • Yaned M. Correa
  • Jaime Niño
  • Oscar M. Mosquera

Abstract

Callos de Taxus baccata fueron inducidos a partir de segmentos de agujas y tallos en el medio de cultivo TM5 suplementado con diferentes concentraciones de picloram en combinación con ácido giberélico (GA3) y kinetina. Los segmentos de tallo produjeron callos con tasas de crecimiento más altas que las obtenidas a partir de las agujas. Los mejores medios para la inducción de callos fueron TM5 suplementados con 1 o 6 mg/L de picloram en combinación con 0,1 mg/L de GA3 y 0,1 mg/L de kinetina respectivamente. El análisis por HPLC de los extractos metanólicos de los callos determinó que el rango de concentración de Taxol estuvo entre 1,90–8,75 mg/L y el de Baccatin III entre 4,55–18,90 mg/L.

Downloads

Download data is not yet available.

Downloads

Published

2006-01-12

How to Cite

Correa, Y. M., Niño, J., & Mosquera, O. M. (2006). Cuantificacion de taxol y baccatin iii en callos de taxus baccata por cromatografia liquida de alta eficiencia (hplc). Scientia Et Technica, 3(32). Retrieved from https://revistas.utp.edu.co/index.php/revistaciencia/article/view/6335

Issue

Vol. 3 No. 32 (2006)

Section

Ciencias Básicas

License

Copyright (c) 2017 Scientia et technica

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Copyrights

The journal is free open access. The papers are published under the Creative Commons Attribution / Attribution-NonCommercial-NoDerivatives 4.0 International - CC BY-NC-ND 4.0 license. For this reason, the author or authors of a manuscript accepted for publication will yield all the economic rights to the Universidad Tecnológica of Pereira free of charge, taking into account the following:

In the event that the submitted manuscript is accepted for publication, the authors must grant permission to the journal, in unlimited time, to reproduce, to edit, distribute, exhibit and publish anywhere, either by means printed, electronic, databases, repositories, optical discs, Internet or any other required medium. In all cases, the journal preserves the obligation to respect, the moral rights of the authors, contained in article 30 of Law 23 of 1982 of the Government Colombian.

The transferors using ASSIGNMENT OF PATRIMONIAL RIGHTS letter declare that all the material that is part of the article is entirely free of copyright.  Therefore, the authors are responsible for any litigation or related claim to intellectual property rights. They exonerate of all responsibility to the Universidad Tecnológica of Pereira (publishing entity) and the Scientia et Technica journal. Likewise, the authors accept that the work presented will be distributed in free open access, safeguarding copyright under the Creative Commons Attribution / Recognition-NonCommercial-NoDerivatives 4.0 International - https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es license.